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anti cd205  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti cd205
    Anti Cd205, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-cd205/anti+cd86/pm40299654-84-5-6
    Average 90 stars, based on 1 article reviews
    anti cd205 - by Bioz Stars, 2026-09
    90/100 stars

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    Staining:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Marker:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Purification:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Recombinant:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Plasmid Preparation:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Microscopy:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Software:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Flow Cytometry:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    FACS:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Incubation:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Real-time Polymerase Chain Reaction:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Blocking Assay:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Expressing:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Functional Assay:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Mutagenesis:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Western Blot:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Injection:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Isolation:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Quantitative RT-PCR:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Activity Assay:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    BrdU Incorporation Assay:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Immunofluorescence:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Sequencing:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Binding Assay:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Transfection:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Luciferase:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Negative Control:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.

    Over Expression:

    Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
    Article Snippet: The following antibodies were used: anti-IgD (11–26c.2a; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; BD Bioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (HL3; BD Biosciences), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL International), anti-fibroblasts monoclonal antibody (ER-TR-7; Thermo Fisher Scientific), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; Thermo Fisher Scientific), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti- Salmonella (Polyclonal; Abcam).

    Article Title: Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r .com/ locate / th romres Regular Article Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia Xiao-Lin Zhang a,⁎, Ji Ma a, Miao Xu a, Fanli Meng a, Mingming Qu a, Jianzhi Sun a, Ping Qin a, Lin Wang a, Yu Hou a, Qiang Song a, Jun Peng a,b, Ming Hou a,b a Department of Hematology, Qilu Hospital, Shandong University, Jinan, China b Key Laboratory of Cardiovascular Remodelling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, China ⁎ Corresponding author at: Department of Hemato University, 107 West Wenhua Road, Jinan, Shandong 82169861; fax: +86 531 86927544.. E-mail address: sunnyzxl@126.com (X.-L. Zhang). http://dx.doi.org/10.1016/j.thromres.2014.11.042 0049-3848/© 2014 Elsevier Ltd. All rights reserved. a b s t r a c t a r t i c l e i n f o

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Antibodies for Kit, CD25, CD3ε, CD4, CD8, MHCII, CD40, CD205, CD80, CD45, Ly5.1, Ly5.2, Ly51, Mac1, Ter119, B220, Gr1, and Sca1 were purchased from eBioscience.

    Article Title: Alternative NF-κB signaling regulates mTEC differentiation from podoplanin-expressing precursors in the cortico-medullary junction.
    Article Snippet: Flow cytometry and cell sorting CD45 depleted stromal cell suspensions were incubated for 20 min at 4°C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-CD45, anti-PDPN, anti-CD326, anti-CD205, anti-I-Ab, anti-CD80, and anti-CD249 (all from eBioscience), antiUEA1 (Sigma Aldrich) antibodies.

    Article Title: Preferential induction of CD4+ T cell responses through in vivo targeting of antigen to dendritic cell-associated C-type lectin-1.
    Article Snippet: Isotype control, anti-CD205, and anti-Dectin-1 Abs were conjugated to AlexaFluor 488 and AlexaFluor 647 fluorochromes using Ab labeling kits (Molecular Probes) according to the manufacturer’s protocols.

    Article Title: Salmonella infection induces the reorganisation of follicular dendritic cell networks concomitant with the failure to generate germinal centres
    Article Snippet: The following antibodies were used: anti-IgD (11-26C.1; BD Bioscience), anti-B220 (RA3-6B2), anti-CD3 (145-2C11; eBioscience), anti-laminin (Polyclonal; Invitrogen), anti-CD209b (SIGN-R1; 22D1; eBioscience), anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta; eBioscience), anti-CD11c (N418; BioLegend), anti-IgM (Polyclonal; Southern Biotech), anti-milk fat globule epidermal growth factor 8 protein (Mfge-8; 18A2-G10; MBL), anti-fibroblasts monoclonal antibody (ER-TR-7; Invitrogen), anti-follicular dendritic cell (FDC-M1; BD Bioscience), anti-CD21/35 (REA800; Miltenyi Biotec), anti-CD31 (Polyclonal; R&D System), anti-PDGFRβ (APB5; eBioscience), anti-CCL21 (Polyclonal; R&D System) and anti-CXCL13 (Polyclonal; R&D System), anti-MadCAM-1 (MECA-367; eBioscience), anti-Ki-67 (Polyclonal; Abcam), peanut agglutinin (PNA; Vector Laboratories), and anti-Salmonella (Polyclonal; Abcam).

    Article Title: The alarmin IL33 orchestrates type 2 immune-mediated control of thymus regeneration
    Article Snippet: Sections were stained with the following antibodies: mTEC marker ERTR5 , anti-Aire (5H12, Invitrogen), anti-CD205 (205yekta, Invitrogen).

    Article Title: Cowpox Virus Inhibits Human Dendritic Cell Immune Function By Nonlethal, Nonproductive Infection
    Article Snippet: Suspensions of purified MDDCs and mDCs were incubated with FcR blocking reagent (Miltenyi Biotec) and stained with the mAbs anti-CD14-FITC (M5E2, mouse IgG2a), anti-HLA-DR-PerCP (L243, mouse IgG2a), anti-CD11c-APC (B-ly6, mouse IgG1) and a PE-conjugated mAb for surface molecule expression or controls as follows: anti-CD86 (IT2.2, mouse IgG2b), anti-CD80 (L307.4, mouse IgG1), anti-CD40 (5C3, mouse IgG1), mouse IgG1 (MOPC-21), mouse IgG2b (27-35) or with biotinylated CD205 (DEC205) (MG 38, mouse IgG2b, eBiosciences, San Diego, CA), mouse IgG2b (ebmG2b, eBiosciences), followed by streptavidin-PE for detection.



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    Image Search Results


    Example of a 15-color antibody panel (plus autofluorescence) and their respective dilutions

    Journal: STAR Protocols

    Article Title: Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context

    doi: 10.1016/j.xpro.2023.102601

    Figure Lengend Snippet: Example of a 15-color antibody panel (plus autofluorescence) and their respective dilutions

    Article Snippet: Anti-CD205 – Super Bright 436 (205yekta), 1:40 , Thermo Fisher Scientific , RRID: AB_2637130.

    Techniques:

    Journal: STAR Protocols

    Article Title: Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context

    doi: 10.1016/j.xpro.2023.102601

    Figure Lengend Snippet:

    Article Snippet: Anti-CD205 – Super Bright 436 (205yekta), 1:40 , Thermo Fisher Scientific , RRID: AB_2637130.

    Techniques: Western Blot, Recombinant, Software, Fluorescence, Microscopy